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To pool or not to pool samples for sexually transmitted infections detection in men who have sex with men? An evaluation of a new pooling method using the GeneXpert instrument in West-Africa. / CohMSM-PrEP pooling study group.

In: Sexually Transmitted Diseases, Vol. 47, Nr. 8, 2020, blz. 556-561.

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@article{83ad73763fb14a85b12fb9c8a573f09b,
title = "To pool or not to pool samples for sexually transmitted infections detection in men who have sex with men? An evaluation of a new pooling method using the GeneXpert instrument in West-Africa",
abstract = "BACKGROUND: Men who have sex with men (MSM) using preexposure prophylaxis (PrEP) are at risk for sexually transmitted infections (STIs). Therefore, PrEP services should include regular screening for Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) at urethra, anorectum, and pharynx. However, financial and logistic challenges arise in low-resource settings. We assessed a new STI sample pooling method using the GeneXpert instrument among MSM initiating PrEP in West Africa.METHODS: Urine, anorectal, and pharyngeal samples were pooled per individual for analysis. In case of an invalid result only (strategy 1) or a positive result of the pool (strategy 2), samples were analyzed individually to identify the infection's biological location. The results of 2 different pooling strategies were compared against the individual results obtained by a criterion standard.RESULTS: We found a prevalence of 14.5{\%} for chlamydia and 11.5{\%} for gonorrhea, with a predominance of infections being extragenital (77.6{\%}). The majority of infections were asymptomatic (88.2{\%}). The pooling strategy 1, had a sensitivity, specificity and agreement for CT of 95.4{\%}, 98.7{\%}, and 0.93, respectively; and 92.3{\%}, 99.2{\%}, and 0.93 for pooling strategy 2. For NG, these figures were 88.9{\%}, 97.7{\%}, and 0.85 for strategy 1, and 88.9{\%}, 96.7{\%}, and 0.81 for strategy 2.CONCLUSIONS: West African MSM have a high prevalence of extragenital and asymptomatic STIs. The GeneXpert method provides an opportunity to move from syndromic toward etiological STI diagnosis in low-income countries, as the platform is available in African countries for tuberculosis testing. Pooling will reduce costs of triple site testing.",
author = "{CohMSM-PrEP pooling study group} and {De Baetselier}, Irith and Bea Vuylsteke and Issifou Yaya and Anoumou Dagnra and Souba Diand{\'e} and Ephrem Mensah and Elias Dah and Camille Anoma and Amadou Kon{\'e} and Hortense Fay{\'e}-Kett{\'e} and Alain Yeo and Keita, {Bintou Demb{\'e}l{\'e}} and Christian Laurent and Tania Crucitti",
note = "CPDF",
year = "2020",
doi = "10.1097/OLQ.0000000000001191",
language = "English",
volume = "47",
pages = "556--561",
journal = "Sexually Transmitted Diseases",
issn = "0148-5717",
publisher = "Lippincott Williams & Wilkins",
number = "8",

}

RIS

TY - JOUR

T1 - To pool or not to pool samples for sexually transmitted infections detection in men who have sex with men? An evaluation of a new pooling method using the GeneXpert instrument in West-Africa

AU - CohMSM-PrEP pooling study group

AU - De Baetselier, Irith

AU - Vuylsteke, Bea

AU - Yaya, Issifou

AU - Dagnra, Anoumou

AU - Diandé, Souba

AU - Mensah, Ephrem

AU - Dah, Elias

AU - Anoma, Camille

AU - Koné, Amadou

AU - Fayé-Ketté, Hortense

AU - Yeo, Alain

AU - Keita, Bintou Dembélé

AU - Laurent, Christian

AU - Crucitti, Tania

N1 - CPDF

PY - 2020

Y1 - 2020

N2 - BACKGROUND: Men who have sex with men (MSM) using preexposure prophylaxis (PrEP) are at risk for sexually transmitted infections (STIs). Therefore, PrEP services should include regular screening for Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) at urethra, anorectum, and pharynx. However, financial and logistic challenges arise in low-resource settings. We assessed a new STI sample pooling method using the GeneXpert instrument among MSM initiating PrEP in West Africa.METHODS: Urine, anorectal, and pharyngeal samples were pooled per individual for analysis. In case of an invalid result only (strategy 1) or a positive result of the pool (strategy 2), samples were analyzed individually to identify the infection's biological location. The results of 2 different pooling strategies were compared against the individual results obtained by a criterion standard.RESULTS: We found a prevalence of 14.5% for chlamydia and 11.5% for gonorrhea, with a predominance of infections being extragenital (77.6%). The majority of infections were asymptomatic (88.2%). The pooling strategy 1, had a sensitivity, specificity and agreement for CT of 95.4%, 98.7%, and 0.93, respectively; and 92.3%, 99.2%, and 0.93 for pooling strategy 2. For NG, these figures were 88.9%, 97.7%, and 0.85 for strategy 1, and 88.9%, 96.7%, and 0.81 for strategy 2.CONCLUSIONS: West African MSM have a high prevalence of extragenital and asymptomatic STIs. The GeneXpert method provides an opportunity to move from syndromic toward etiological STI diagnosis in low-income countries, as the platform is available in African countries for tuberculosis testing. Pooling will reduce costs of triple site testing.

AB - BACKGROUND: Men who have sex with men (MSM) using preexposure prophylaxis (PrEP) are at risk for sexually transmitted infections (STIs). Therefore, PrEP services should include regular screening for Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) at urethra, anorectum, and pharynx. However, financial and logistic challenges arise in low-resource settings. We assessed a new STI sample pooling method using the GeneXpert instrument among MSM initiating PrEP in West Africa.METHODS: Urine, anorectal, and pharyngeal samples were pooled per individual for analysis. In case of an invalid result only (strategy 1) or a positive result of the pool (strategy 2), samples were analyzed individually to identify the infection's biological location. The results of 2 different pooling strategies were compared against the individual results obtained by a criterion standard.RESULTS: We found a prevalence of 14.5% for chlamydia and 11.5% for gonorrhea, with a predominance of infections being extragenital (77.6%). The majority of infections were asymptomatic (88.2%). The pooling strategy 1, had a sensitivity, specificity and agreement for CT of 95.4%, 98.7%, and 0.93, respectively; and 92.3%, 99.2%, and 0.93 for pooling strategy 2. For NG, these figures were 88.9%, 97.7%, and 0.85 for strategy 1, and 88.9%, 96.7%, and 0.81 for strategy 2.CONCLUSIONS: West African MSM have a high prevalence of extragenital and asymptomatic STIs. The GeneXpert method provides an opportunity to move from syndromic toward etiological STI diagnosis in low-income countries, as the platform is available in African countries for tuberculosis testing. Pooling will reduce costs of triple site testing.

U2 - 10.1097/OLQ.0000000000001191

DO - 10.1097/OLQ.0000000000001191

M3 - A1: Web of Science-article

C2 - 32355106

VL - 47

SP - 556

EP - 561

JO - Sexually Transmitted Diseases

JF - Sexually Transmitted Diseases

SN - 0148-5717

IS - 8

ER -

ID: 3341553