• CohMSM-PrEP pooling study group

BACKGROUND: Men who have sex with Men (MSM) using Pre-exposure prophylaxis (PrEP) are at risk for Sexually Transmitted Infections (STIs). Therefore, PrEP services should include regular screening for Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) at urethra, anorectum and pharynx. However, financial and logistic challenges arise in low resource settings. We assessed a new STI sample pooling method using the GeneXpert instrument among MSM initiating PrEP in West-Africa.

METHODS: Urine, anorectal and pharyngeal samples were pooled per individual for analysis. In case of an invalid result only (strategy one) or a positive result of the pool (strategy two), samples were analyzed individually to identify the infection's biological location. The results of two different pooling strategies were compared against the individual results obtained by a gold standard RESULTS: We found a prevalence of 14.5% for chlamydia and 11.5% for gonorrhea, with a predominance of infections being extra-genital (77.6%). The majority of infections were asymptomatic (88.2%). The pooling strategy one, had a sensitivity, specificity and agreement for CT of 95.4%, 98.7% and 0.93, respectively; and 92.3%, 99.2% and 0.93 for pooling strategy two. For NG, these figures were 88.9%, 97.7% and 0.85 for strategy one, and 88.9%, 96.7% and 0.81 for strategy two.

CONCLUSIONS: West-African MSM have a high prevalence of extra-genital and asymptomatic STIs. The GeneXpert method provides an opportunity to move from syndromic towards etiological STI diagnosis in low income countries, as the platform is available in African countries for tuberculosis testing. Pooling will reduce costs of triple site testing.

Originele taal-2Engels
TijdschriftSexually Transmitted Diseases
ISSN0148-5717
DOI's
StatusE-publicatie voorafgaand op geprinte versie - 29-apr-2020

ID: 3341553