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Significant under expression of the DosR regulon in M. tuberculosis complex lineage 6 in sputum. / Ofori-Anyinam, Boatema; Dolganov, Gregory; Van, Tran; Davis, J Lucian; Walter, Nicholas D; Garcia, Benjamin J; Voskuil, Marty; Fissette, Kristina; Diels, Maren; Driesen, Michèle; Meehan, Conor J; Yeboah-Manu, Dorothy; Coscolla, Mireia; Gagneux, Sebastien; Antonio, Martin; Schoolnik, Gary; Gehre, Florian; de Jong, Bouke C.

In: Tuberculosis, Vol. 104, 2017, blz. 58-64.

Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikel

Harvard

Ofori-Anyinam, B, Dolganov, G, Van, T, Davis, JL, Walter, ND, Garcia, BJ, Voskuil, M, Fissette, K, Diels, M, Driesen, M, Meehan, CJ, Yeboah-Manu, D, Coscolla, M, Gagneux, S, Antonio, M, Schoolnik, G, Gehre, F & de Jong, BC 2017, 'Significant under expression of the DosR regulon in M. tuberculosis complex lineage 6 in sputum', Tuberculosis, vol. 104, blz. 58-64. https://doi.org/10.1016/j.tube.2017.03.001

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Author

Ofori-Anyinam, Boatema ; Dolganov, Gregory ; Van, Tran ; Davis, J Lucian ; Walter, Nicholas D ; Garcia, Benjamin J ; Voskuil, Marty ; Fissette, Kristina ; Diels, Maren ; Driesen, Michèle ; Meehan, Conor J ; Yeboah-Manu, Dorothy ; Coscolla, Mireia ; Gagneux, Sebastien ; Antonio, Martin ; Schoolnik, Gary ; Gehre, Florian ; de Jong, Bouke C. / Significant under expression of the DosR regulon in M. tuberculosis complex lineage 6 in sputum. In: Tuberculosis. 2017 ; Vol. 104. blz. 58-64.

BibTeX

@article{ffca1cc56d6543a9b3797a4eb5a9dc51,
title = "Significant under expression of the DosR regulon in M. tuberculosis complex lineage 6 in sputum",
abstract = "Mycobacterium africanum lineage (L) 6 is an important pathogen in West Africa, causing up to 40{\%} of pulmonary tuberculosis (TB). The biology underlying the clinical differences between M. africanum and M. tuberculosis sensu stricto remains poorly understood. We performed ex vivo expression of 2179 genes of the most geographically dispersed cause of human TB, M. tuberculosis L4 and the geographically restricted, M. africanum L6 directly from sputa of 11 HIV-negative TB patients from The Gambia who had not started treatment. The DosR regulon was the most significantly decreased category in L6 relative to L4. Further, we identified nonsynonymous mutations in major DosR regulon genes of 44 L6 genomes of TB patients from The Gambia and Ghana. Using Lebek's test, we assessed differences in oxygen requirements for growth. L4 grew only at the aerobic surface while L6 grew throughout the medium. In the host, the DosR regulon is critical for M. tuberculosis in adaptation to oxygen limitation. However, M. africanum L6 appears to have adapted to growth under hypoxic conditions or to different biological niches. The observed under expression of DosR in L6 fits with the genomic changes in DosR genes, microaerobic growth and the association with extrapulmonary disease.",
keywords = "Journal Article",
author = "Boatema Ofori-Anyinam and Gregory Dolganov and Tran Van and Davis, {J Lucian} and Walter, {Nicholas D} and Garcia, {Benjamin J} and Marty Voskuil and Kristina Fissette and Maren Diels and Mich{\`e}le Driesen and Meehan, {Conor J} and Dorothy Yeboah-Manu and Mireia Coscolla and Sebastien Gagneux and Martin Antonio and Gary Schoolnik and Florian Gehre and {de Jong}, {Bouke C}",
note = "Copyright {\circledC} 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.",
year = "2017",
doi = "10.1016/j.tube.2017.03.001",
language = "English",
volume = "104",
pages = "58--64",
journal = "Tuberculosis",
issn = "1472-9792",
publisher = "Churchill Livingstone",

}

RIS

TY - JOUR

T1 - Significant under expression of the DosR regulon in M. tuberculosis complex lineage 6 in sputum

AU - Ofori-Anyinam, Boatema

AU - Dolganov, Gregory

AU - Van, Tran

AU - Davis, J Lucian

AU - Walter, Nicholas D

AU - Garcia, Benjamin J

AU - Voskuil, Marty

AU - Fissette, Kristina

AU - Diels, Maren

AU - Driesen, Michèle

AU - Meehan, Conor J

AU - Yeboah-Manu, Dorothy

AU - Coscolla, Mireia

AU - Gagneux, Sebastien

AU - Antonio, Martin

AU - Schoolnik, Gary

AU - Gehre, Florian

AU - de Jong, Bouke C

N1 - Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.

PY - 2017

Y1 - 2017

N2 - Mycobacterium africanum lineage (L) 6 is an important pathogen in West Africa, causing up to 40% of pulmonary tuberculosis (TB). The biology underlying the clinical differences between M. africanum and M. tuberculosis sensu stricto remains poorly understood. We performed ex vivo expression of 2179 genes of the most geographically dispersed cause of human TB, M. tuberculosis L4 and the geographically restricted, M. africanum L6 directly from sputa of 11 HIV-negative TB patients from The Gambia who had not started treatment. The DosR regulon was the most significantly decreased category in L6 relative to L4. Further, we identified nonsynonymous mutations in major DosR regulon genes of 44 L6 genomes of TB patients from The Gambia and Ghana. Using Lebek's test, we assessed differences in oxygen requirements for growth. L4 grew only at the aerobic surface while L6 grew throughout the medium. In the host, the DosR regulon is critical for M. tuberculosis in adaptation to oxygen limitation. However, M. africanum L6 appears to have adapted to growth under hypoxic conditions or to different biological niches. The observed under expression of DosR in L6 fits with the genomic changes in DosR genes, microaerobic growth and the association with extrapulmonary disease.

AB - Mycobacterium africanum lineage (L) 6 is an important pathogen in West Africa, causing up to 40% of pulmonary tuberculosis (TB). The biology underlying the clinical differences between M. africanum and M. tuberculosis sensu stricto remains poorly understood. We performed ex vivo expression of 2179 genes of the most geographically dispersed cause of human TB, M. tuberculosis L4 and the geographically restricted, M. africanum L6 directly from sputa of 11 HIV-negative TB patients from The Gambia who had not started treatment. The DosR regulon was the most significantly decreased category in L6 relative to L4. Further, we identified nonsynonymous mutations in major DosR regulon genes of 44 L6 genomes of TB patients from The Gambia and Ghana. Using Lebek's test, we assessed differences in oxygen requirements for growth. L4 grew only at the aerobic surface while L6 grew throughout the medium. In the host, the DosR regulon is critical for M. tuberculosis in adaptation to oxygen limitation. However, M. africanum L6 appears to have adapted to growth under hypoxic conditions or to different biological niches. The observed under expression of DosR in L6 fits with the genomic changes in DosR genes, microaerobic growth and the association with extrapulmonary disease.

KW - Journal Article

U2 - 10.1016/j.tube.2017.03.001

DO - 10.1016/j.tube.2017.03.001

M3 - A1: Web of Science-article

C2 - 28454650

VL - 104

SP - 58

EP - 64

JO - Tuberculosis

JF - Tuberculosis

SN - 1472-9792

ER -

ID: 2073980