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Increased KLRG1 and PD-1 expression on CD8 T lymphocytes in TB-IRIS. / TB-IRIS Study Grp.

In: PLoS ONE, Vol. 14, Nr. 4, 0215991, 2019.

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TB-IRIS Study Grp. / Increased KLRG1 and PD-1 expression on CD8 T lymphocytes in TB-IRIS. In: PLoS ONE. 2019 ; Vol. 14, Nr. 4.

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@article{6c4d27c033f7405395de93e47bd95b4f,
title = "Increased KLRG1 and PD-1 expression on CD8 T lymphocytes in TB-IRIS",
abstract = "BackgroundTuberculosis-associated immune reconstitution inflammatory syndrome (TB-IRIS) is an inflammatory complication in HIV-TB co-infected patients receiving antiretroviral therapy (ART). The exact contribution of T cells, natural killer (NK) cells, and monocytes to TB-IRIS development remains unclear. Here, we studied the expression of exhaustion markers on lymphocytes at different intervals during ART.MethodsWe compared 13 HIV-TB patients who developed TB-IRIS with 13 patients who did not (HIV+TB+), 13 HIV-patients without TB (HIV+TB-) and 9 HIV/TB-negative controls (HIV-TB-). Patients did not differ in age, gender, or CD4-count prior to ART. Frozen peripheral blood mononuclear cells, collected before ART and during 3 months and 9 months of ART, were analysed using flow cytometry. We examined expression of KLRG1, PD-1 and IL-27R on CD4(+) and CD8(hi) T cells, as well as CD3-negative CD8(lo) lymphocytes as an approximate subset of NK cells. In addition, expression of TLR2, TLR4, IL1RL1, and TRAILR on CD14(+) monocytes were investigated.ResultsPrior to ART, TB-IRIS patients had higher percentages of CD8(hi) T cells that are KLRG1(+)PD-1(+) compared to each control group (pConclusionTB-IRIS is preceded by a high level of exhausted (KLRG1(+)PD-1(+)) CD8(hi) T cells, which persists during 3 months of ART. This trait is potentially mirrored in a subpopulation of NK cells, but not CD4(+) T cells. Since a dysfunctional CD8(+) lymphocyte compartment could predispose patients to TB-IRIS, the functional role of these cells prior to TB-IRIS development should be further explored.",
keywords = "RECONSTITUTION INFLAMMATORY SYNDROME, HIV-INFECTED PATIENTS, ANTIRETROVIRAL THERAPY, TUBERCULOSIS, CELLS, DISEASE",
author = "{TB-IRIS Study Grp} and Odin Goovaerts and Marguerite Massinga-Loembe and Pascale Ondoa and Ann Ceulemans and William Worodria and Harriet Mayanja and Robert Colebunders and Luc Kestens and Luc Kestens",
note = "CPDF; CUNIT",
year = "2019",
doi = "10.1371/journal.pone.0215991",
language = "English",
volume = "14",
journal = "PLoS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "4",

}

RIS

TY - JOUR

T1 - Increased KLRG1 and PD-1 expression on CD8 T lymphocytes in TB-IRIS

AU - TB-IRIS Study Grp

AU - Goovaerts, Odin

AU - Massinga-Loembe, Marguerite

AU - Ondoa, Pascale

AU - Ceulemans, Ann

AU - Worodria, William

AU - Mayanja, Harriet

AU - Colebunders, Robert

AU - Kestens, Luc

AU - Kestens, Luc

N1 - CPDF; CUNIT

PY - 2019

Y1 - 2019

N2 - BackgroundTuberculosis-associated immune reconstitution inflammatory syndrome (TB-IRIS) is an inflammatory complication in HIV-TB co-infected patients receiving antiretroviral therapy (ART). The exact contribution of T cells, natural killer (NK) cells, and monocytes to TB-IRIS development remains unclear. Here, we studied the expression of exhaustion markers on lymphocytes at different intervals during ART.MethodsWe compared 13 HIV-TB patients who developed TB-IRIS with 13 patients who did not (HIV+TB+), 13 HIV-patients without TB (HIV+TB-) and 9 HIV/TB-negative controls (HIV-TB-). Patients did not differ in age, gender, or CD4-count prior to ART. Frozen peripheral blood mononuclear cells, collected before ART and during 3 months and 9 months of ART, were analysed using flow cytometry. We examined expression of KLRG1, PD-1 and IL-27R on CD4(+) and CD8(hi) T cells, as well as CD3-negative CD8(lo) lymphocytes as an approximate subset of NK cells. In addition, expression of TLR2, TLR4, IL1RL1, and TRAILR on CD14(+) monocytes were investigated.ResultsPrior to ART, TB-IRIS patients had higher percentages of CD8(hi) T cells that are KLRG1(+)PD-1(+) compared to each control group (pConclusionTB-IRIS is preceded by a high level of exhausted (KLRG1(+)PD-1(+)) CD8(hi) T cells, which persists during 3 months of ART. This trait is potentially mirrored in a subpopulation of NK cells, but not CD4(+) T cells. Since a dysfunctional CD8(+) lymphocyte compartment could predispose patients to TB-IRIS, the functional role of these cells prior to TB-IRIS development should be further explored.

AB - BackgroundTuberculosis-associated immune reconstitution inflammatory syndrome (TB-IRIS) is an inflammatory complication in HIV-TB co-infected patients receiving antiretroviral therapy (ART). The exact contribution of T cells, natural killer (NK) cells, and monocytes to TB-IRIS development remains unclear. Here, we studied the expression of exhaustion markers on lymphocytes at different intervals during ART.MethodsWe compared 13 HIV-TB patients who developed TB-IRIS with 13 patients who did not (HIV+TB+), 13 HIV-patients without TB (HIV+TB-) and 9 HIV/TB-negative controls (HIV-TB-). Patients did not differ in age, gender, or CD4-count prior to ART. Frozen peripheral blood mononuclear cells, collected before ART and during 3 months and 9 months of ART, were analysed using flow cytometry. We examined expression of KLRG1, PD-1 and IL-27R on CD4(+) and CD8(hi) T cells, as well as CD3-negative CD8(lo) lymphocytes as an approximate subset of NK cells. In addition, expression of TLR2, TLR4, IL1RL1, and TRAILR on CD14(+) monocytes were investigated.ResultsPrior to ART, TB-IRIS patients had higher percentages of CD8(hi) T cells that are KLRG1(+)PD-1(+) compared to each control group (pConclusionTB-IRIS is preceded by a high level of exhausted (KLRG1(+)PD-1(+)) CD8(hi) T cells, which persists during 3 months of ART. This trait is potentially mirrored in a subpopulation of NK cells, but not CD4(+) T cells. Since a dysfunctional CD8(+) lymphocyte compartment could predispose patients to TB-IRIS, the functional role of these cells prior to TB-IRIS development should be further explored.

KW - RECONSTITUTION INFLAMMATORY SYNDROME

KW - HIV-INFECTED PATIENTS

KW - ANTIRETROVIRAL THERAPY

KW - TUBERCULOSIS

KW - CELLS

KW - DISEASE

U2 - 10.1371/journal.pone.0215991

DO - 10.1371/journal.pone.0215991

M3 - A1: Web of Science-article

VL - 14

JO - PLoS ONE

T2 - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 4

M1 - 0215991

ER -

ID: 2955037