Standard

First international external quality assessment scheme of nucleic acid amplification tests for the detection of Schistosoma and soil-transmitted helminths, including Strongyloides: a pilot study. / Cools, Piet; van Lieshout, Lisette; Koelewijn, Rob; Addiss, David; Ajjampur, Sitara S R; Ayana, Mio; Bradbury, Richard S; Cantera, Jason L; Dana, Daniel; Fischer, Kerstin; Imtiaz, Rubina; Kabagenyi, Joyce; Lok, James; McCarthy, James; Mejia, Rojelio; Mekonnen, Zeleke; Njenga, Sammy M; Othman, Nurulhasanah; Shao, Hongguang; Traub, Rebecca; Van Esbroeck, Marjan; Vercruysse, Jozef; Vlaminck, Johnny; Williams, Steven A; Verweij, Jaco J; van Hellemond, Jaap J; Levecke, Bruno.

In: PLoS Neglected Tropical Diseases, Vol. 14, Nr. 6, 2020, blz. e0008231.

Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikel

Harvard

Cools, P, van Lieshout, L, Koelewijn, R, Addiss, D, Ajjampur, SSR, Ayana, M, Bradbury, RS, Cantera, JL, Dana, D, Fischer, K, Imtiaz, R, Kabagenyi, J, Lok, J, McCarthy, J, Mejia, R, Mekonnen, Z, Njenga, SM, Othman, N, Shao, H, Traub, R, Van Esbroeck, M, Vercruysse, J, Vlaminck, J, Williams, SA, Verweij, JJ, van Hellemond, JJ & Levecke, B 2020, 'First international external quality assessment scheme of nucleic acid amplification tests for the detection of Schistosoma and soil-transmitted helminths, including Strongyloides: a pilot study', PLoS Neglected Tropical Diseases, vol. 14, nr. 6, blz. e0008231. https://doi.org/10.1371/journal.pntd.0008231

APA

Cools, P., van Lieshout, L., Koelewijn, R., Addiss, D., Ajjampur, S. S. R., Ayana, M., ... Levecke, B. (2020). First international external quality assessment scheme of nucleic acid amplification tests for the detection of Schistosoma and soil-transmitted helminths, including Strongyloides: a pilot study. PLoS Neglected Tropical Diseases, 14(6), e0008231. https://doi.org/10.1371/journal.pntd.0008231

Vancouver

Author

Cools, Piet ; van Lieshout, Lisette ; Koelewijn, Rob ; Addiss, David ; Ajjampur, Sitara S R ; Ayana, Mio ; Bradbury, Richard S ; Cantera, Jason L ; Dana, Daniel ; Fischer, Kerstin ; Imtiaz, Rubina ; Kabagenyi, Joyce ; Lok, James ; McCarthy, James ; Mejia, Rojelio ; Mekonnen, Zeleke ; Njenga, Sammy M ; Othman, Nurulhasanah ; Shao, Hongguang ; Traub, Rebecca ; Van Esbroeck, Marjan ; Vercruysse, Jozef ; Vlaminck, Johnny ; Williams, Steven A ; Verweij, Jaco J ; van Hellemond, Jaap J ; Levecke, Bruno. / First international external quality assessment scheme of nucleic acid amplification tests for the detection of Schistosoma and soil-transmitted helminths, including Strongyloides: a pilot study. In: PLoS Neglected Tropical Diseases. 2020 ; Vol. 14, Nr. 6. blz. e0008231.

BibTeX

@article{06e78cb6998a46daba6c66e40b4a67b9,
title = "First international external quality assessment scheme of nucleic acid amplification tests for the detection of Schistosoma and soil-transmitted helminths, including Strongyloides: a pilot study",
abstract = "BACKGROUND: Nucleic acid amplification tests (NAATs) are increasingly being used as diagnostic tools for soil-transmitted helminths (STHs; Ascaris lumbricoides, Trichuris trichiura, Necator americanus, Ancylostoma duodenale and A. ceylanicum), Strongyloides stercoralis and Schistosoma in human stool. Currently, there is a large diversity of NAATs being applied, but an external quality assessment scheme (EQAS) for these diagnostics is lacking. An EQAS involves a blinded process where test results reported by a laboratory are compared to those reported by reference or expert laboratories, allowing for an objective assessment of the diagnostic performance of a laboratory. In the current study, we piloted an international EQAS for these helminths (i) to investigate the feasibility of designing and delivering an EQAS; (ii) to assess the diagnostic performance of laboratories; and (iii) to gain insights into the different NAAT protocols used.METHODS AND PRINCIPAL FINDINGS: A panel of twelve stool samples and eight DNA samples was validated by six expert laboratories for the presence of six helminths (Ascaris, Trichuris, N. americanus, Ancylostoma, Strongyloides and Schistosoma). Subsequently this panel was sent to 15 globally dispersed laboratories. We found a high degree of diversity among the different DNA extraction and NAAT protocols. Although most laboratories performed well, we could clearly identify the laboratories that were poorly performing.CONCLUSIONS/SIGNIFICANCE: We showed the technical feasibility of an international EQAS for the NAAT of STHs, Strongyloides and Schistosoma. In addition, we documented that there are clear benefits for participating laboratories, as they can confirm and/or improve the diagnostic performance of their NAATs. Further research should aim to identify factors that explain poor performance of NAATs.",
author = "Piet Cools and {van Lieshout}, Lisette and Rob Koelewijn and David Addiss and Ajjampur, {Sitara S R} and Mio Ayana and Bradbury, {Richard S} and Cantera, {Jason L} and Daniel Dana and Kerstin Fischer and Rubina Imtiaz and Joyce Kabagenyi and James Lok and James McCarthy and Rojelio Mejia and Zeleke Mekonnen and Njenga, {Sammy M} and Nurulhasanah Othman and Hongguang Shao and Rebecca Traub and {Van Esbroeck}, Marjan and Jozef Vercruysse and Johnny Vlaminck and Williams, {Steven A} and Verweij, {Jaco J} and {van Hellemond}, {Jaap J} and Bruno Levecke",
note = "CPDF",
year = "2020",
doi = "10.1371/journal.pntd.0008231",
language = "English",
volume = "14",
pages = "e0008231",
journal = "PLoS Neglected Tropical Diseases",
issn = "1935-2727",
publisher = "Public Library of Science",
number = "6",

}

RIS

TY - JOUR

T1 - First international external quality assessment scheme of nucleic acid amplification tests for the detection of Schistosoma and soil-transmitted helminths, including Strongyloides: a pilot study

AU - Cools, Piet

AU - van Lieshout, Lisette

AU - Koelewijn, Rob

AU - Addiss, David

AU - Ajjampur, Sitara S R

AU - Ayana, Mio

AU - Bradbury, Richard S

AU - Cantera, Jason L

AU - Dana, Daniel

AU - Fischer, Kerstin

AU - Imtiaz, Rubina

AU - Kabagenyi, Joyce

AU - Lok, James

AU - McCarthy, James

AU - Mejia, Rojelio

AU - Mekonnen, Zeleke

AU - Njenga, Sammy M

AU - Othman, Nurulhasanah

AU - Shao, Hongguang

AU - Traub, Rebecca

AU - Van Esbroeck, Marjan

AU - Vercruysse, Jozef

AU - Vlaminck, Johnny

AU - Williams, Steven A

AU - Verweij, Jaco J

AU - van Hellemond, Jaap J

AU - Levecke, Bruno

N1 - CPDF

PY - 2020

Y1 - 2020

N2 - BACKGROUND: Nucleic acid amplification tests (NAATs) are increasingly being used as diagnostic tools for soil-transmitted helminths (STHs; Ascaris lumbricoides, Trichuris trichiura, Necator americanus, Ancylostoma duodenale and A. ceylanicum), Strongyloides stercoralis and Schistosoma in human stool. Currently, there is a large diversity of NAATs being applied, but an external quality assessment scheme (EQAS) for these diagnostics is lacking. An EQAS involves a blinded process where test results reported by a laboratory are compared to those reported by reference or expert laboratories, allowing for an objective assessment of the diagnostic performance of a laboratory. In the current study, we piloted an international EQAS for these helminths (i) to investigate the feasibility of designing and delivering an EQAS; (ii) to assess the diagnostic performance of laboratories; and (iii) to gain insights into the different NAAT protocols used.METHODS AND PRINCIPAL FINDINGS: A panel of twelve stool samples and eight DNA samples was validated by six expert laboratories for the presence of six helminths (Ascaris, Trichuris, N. americanus, Ancylostoma, Strongyloides and Schistosoma). Subsequently this panel was sent to 15 globally dispersed laboratories. We found a high degree of diversity among the different DNA extraction and NAAT protocols. Although most laboratories performed well, we could clearly identify the laboratories that were poorly performing.CONCLUSIONS/SIGNIFICANCE: We showed the technical feasibility of an international EQAS for the NAAT of STHs, Strongyloides and Schistosoma. In addition, we documented that there are clear benefits for participating laboratories, as they can confirm and/or improve the diagnostic performance of their NAATs. Further research should aim to identify factors that explain poor performance of NAATs.

AB - BACKGROUND: Nucleic acid amplification tests (NAATs) are increasingly being used as diagnostic tools for soil-transmitted helminths (STHs; Ascaris lumbricoides, Trichuris trichiura, Necator americanus, Ancylostoma duodenale and A. ceylanicum), Strongyloides stercoralis and Schistosoma in human stool. Currently, there is a large diversity of NAATs being applied, but an external quality assessment scheme (EQAS) for these diagnostics is lacking. An EQAS involves a blinded process where test results reported by a laboratory are compared to those reported by reference or expert laboratories, allowing for an objective assessment of the diagnostic performance of a laboratory. In the current study, we piloted an international EQAS for these helminths (i) to investigate the feasibility of designing and delivering an EQAS; (ii) to assess the diagnostic performance of laboratories; and (iii) to gain insights into the different NAAT protocols used.METHODS AND PRINCIPAL FINDINGS: A panel of twelve stool samples and eight DNA samples was validated by six expert laboratories for the presence of six helminths (Ascaris, Trichuris, N. americanus, Ancylostoma, Strongyloides and Schistosoma). Subsequently this panel was sent to 15 globally dispersed laboratories. We found a high degree of diversity among the different DNA extraction and NAAT protocols. Although most laboratories performed well, we could clearly identify the laboratories that were poorly performing.CONCLUSIONS/SIGNIFICANCE: We showed the technical feasibility of an international EQAS for the NAAT of STHs, Strongyloides and Schistosoma. In addition, we documented that there are clear benefits for participating laboratories, as they can confirm and/or improve the diagnostic performance of their NAATs. Further research should aim to identify factors that explain poor performance of NAATs.

U2 - 10.1371/journal.pntd.0008231

DO - 10.1371/journal.pntd.0008231

M3 - A1: Web of Science-article

C2 - 32544158

VL - 14

SP - e0008231

JO - PLoS Neglected Tropical Diseases

JF - PLoS Neglected Tropical Diseases

SN - 1935-2727

IS - 6

ER -

ID: 3397555