Standard

Cross-utilisation of template RNAs by alphavirus replicases. / Lello, Laura Sandra; Utt, Age; Bartholomeeusen, Koen; Wang, Sainan; Rausalu, Kai; Kendall, Catherine; Coppens, Sandra; Fragkoudis, Rennos; Tuplin, Andrew; Alphey, Luke; Ariën, Kevin K; Merits, Andres.

In: PLoS Pathogens, Vol. 16, Nr. 9, 2020, blz. e1008825.

Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikel

Harvard

Lello, LS, Utt, A, Bartholomeeusen, K, Wang, S, Rausalu, K, Kendall, C, Coppens, S, Fragkoudis, R, Tuplin, A, Alphey, L, Ariën, KK & Merits, A 2020, 'Cross-utilisation of template RNAs by alphavirus replicases', PLoS Pathogens, vol. 16, nr. 9, blz. e1008825. https://doi.org/10.1371/journal.ppat.1008825

APA

Lello, L. S., Utt, A., Bartholomeeusen, K., Wang, S., Rausalu, K., Kendall, C., ... Merits, A. (2020). Cross-utilisation of template RNAs by alphavirus replicases. PLoS Pathogens, 16(9), e1008825. https://doi.org/10.1371/journal.ppat.1008825

Vancouver

Lello LS, Utt A, Bartholomeeusen K, Wang S, Rausalu K, Kendall C et al. Cross-utilisation of template RNAs by alphavirus replicases. PLoS Pathogens. 2020;16(9):e1008825. https://doi.org/10.1371/journal.ppat.1008825

Author

Lello, Laura Sandra ; Utt, Age ; Bartholomeeusen, Koen ; Wang, Sainan ; Rausalu, Kai ; Kendall, Catherine ; Coppens, Sandra ; Fragkoudis, Rennos ; Tuplin, Andrew ; Alphey, Luke ; Ariën, Kevin K ; Merits, Andres. / Cross-utilisation of template RNAs by alphavirus replicases. In: PLoS Pathogens. 2020 ; Vol. 16, Nr. 9. blz. e1008825.

BibTeX

@article{5247d7a2316345a1a16112a2180bd071,
title = "Cross-utilisation of template RNAs by alphavirus replicases",
abstract = "Most alphaviruses (family Togaviridae) including Sindbis virus (SINV) and other human pathogens, are transmitted by arthropods. The first open reading frame in their positive strand RNA genome encodes for the non-structural polyprotein, a precursor to four separate subunits of the replicase. The replicase interacts with cis-acting elements located near the intergenic region and at the ends of the viral RNA genome. A trans-replication assay was developed and used to analyse the template requirements for nine alphavirus replicases. Replicases of alphaviruses of the Semliki Forest virus complex were able to cross-utilize each other's templates as well as those of outgroup alphaviruses. Templates of outgroup alphaviruses, including SINV and the mosquito-specific Eilat virus, were promiscuous; in contrast, their replicases displayed a limited capacity to use heterologous templates, especially in mosquito cells. The determinants important for efficient replication of template RNA were mapped to the 5' region of the genome. For SINV these include the extreme 5'- end of the genome and sequences corresponding to the first stem-loop structure in the 5' untranslated region. Mutations introduced in these elements drastically reduced infectivity of recombinant SINV genomes. The trans-replicase tools and approaches developed here can be instrumental in studying alphavirus recombination and evolution, but can also be applied to study other viruses such as picornaviruses, flaviviruses and coronaviruses.",
author = "Lello, {Laura Sandra} and Age Utt and Koen Bartholomeeusen and Sainan Wang and Kai Rausalu and Catherine Kendall and Sandra Coppens and Rennos Fragkoudis and Andrew Tuplin and Luke Alphey and Ari{\"e}n, {Kevin K} and Andres Merits",
note = "CPDF",
year = "2020",
doi = "10.1371/journal.ppat.1008825",
language = "English",
volume = "16",
pages = "e1008825",
journal = "PLoS Pathogens",
issn = "1553-7366",
publisher = "Public Library of Science",
number = "9",

}

RIS

TY - JOUR

T1 - Cross-utilisation of template RNAs by alphavirus replicases

AU - Lello, Laura Sandra

AU - Utt, Age

AU - Bartholomeeusen, Koen

AU - Wang, Sainan

AU - Rausalu, Kai

AU - Kendall, Catherine

AU - Coppens, Sandra

AU - Fragkoudis, Rennos

AU - Tuplin, Andrew

AU - Alphey, Luke

AU - Ariën, Kevin K

AU - Merits, Andres

N1 - CPDF

PY - 2020

Y1 - 2020

N2 - Most alphaviruses (family Togaviridae) including Sindbis virus (SINV) and other human pathogens, are transmitted by arthropods. The first open reading frame in their positive strand RNA genome encodes for the non-structural polyprotein, a precursor to four separate subunits of the replicase. The replicase interacts with cis-acting elements located near the intergenic region and at the ends of the viral RNA genome. A trans-replication assay was developed and used to analyse the template requirements for nine alphavirus replicases. Replicases of alphaviruses of the Semliki Forest virus complex were able to cross-utilize each other's templates as well as those of outgroup alphaviruses. Templates of outgroup alphaviruses, including SINV and the mosquito-specific Eilat virus, were promiscuous; in contrast, their replicases displayed a limited capacity to use heterologous templates, especially in mosquito cells. The determinants important for efficient replication of template RNA were mapped to the 5' region of the genome. For SINV these include the extreme 5'- end of the genome and sequences corresponding to the first stem-loop structure in the 5' untranslated region. Mutations introduced in these elements drastically reduced infectivity of recombinant SINV genomes. The trans-replicase tools and approaches developed here can be instrumental in studying alphavirus recombination and evolution, but can also be applied to study other viruses such as picornaviruses, flaviviruses and coronaviruses.

AB - Most alphaviruses (family Togaviridae) including Sindbis virus (SINV) and other human pathogens, are transmitted by arthropods. The first open reading frame in their positive strand RNA genome encodes for the non-structural polyprotein, a precursor to four separate subunits of the replicase. The replicase interacts with cis-acting elements located near the intergenic region and at the ends of the viral RNA genome. A trans-replication assay was developed and used to analyse the template requirements for nine alphavirus replicases. Replicases of alphaviruses of the Semliki Forest virus complex were able to cross-utilize each other's templates as well as those of outgroup alphaviruses. Templates of outgroup alphaviruses, including SINV and the mosquito-specific Eilat virus, were promiscuous; in contrast, their replicases displayed a limited capacity to use heterologous templates, especially in mosquito cells. The determinants important for efficient replication of template RNA were mapped to the 5' region of the genome. For SINV these include the extreme 5'- end of the genome and sequences corresponding to the first stem-loop structure in the 5' untranslated region. Mutations introduced in these elements drastically reduced infectivity of recombinant SINV genomes. The trans-replicase tools and approaches developed here can be instrumental in studying alphavirus recombination and evolution, but can also be applied to study other viruses such as picornaviruses, flaviviruses and coronaviruses.

U2 - 10.1371/journal.ppat.1008825

DO - 10.1371/journal.ppat.1008825

M3 - A1: Web of Science-article

C2 - 32886709

VL - 16

SP - e1008825

JO - PLoS Pathogens

JF - PLoS Pathogens

SN - 1553-7366

IS - 9

ER -

ID: 12495243