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Bayesian evaluation of three serological tests for detecting antibodies against Brucella spp. among humans in the northwestern part of Ecuador. / Ron-Roman, Jorge; Ron-Garrido, Lenin; Abatih, Emmanuel; Celi-Erazo, Maritza; Vizcaino-Ordonez, Laura; Calva-Pacheco, Jaime; Gonzalez-Andrade, Pablo; Berkvens, Dirk; Benitez-Ortiz, Washington; Brandt, Jef; Fretin, David; Saegerman, Claude.

In: American Journal of Tropical Medicine and Hygiene, Vol. 100, Nr. 6, 2019, blz. 1312-1320.

Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikelOnderzoekpeer review

Harvard

Ron-Roman, J, Ron-Garrido, L, Abatih, E, Celi-Erazo, M, Vizcaino-Ordonez, L, Calva-Pacheco, J, Gonzalez-Andrade, P, Berkvens, D, Benitez-Ortiz, W, Brandt, J, Fretin, D & Saegerman, C 2019, 'Bayesian evaluation of three serological tests for detecting antibodies against Brucella spp. among humans in the northwestern part of Ecuador' American Journal of Tropical Medicine and Hygiene, vol. 100, nr. 6, blz. 1312-1320. https://doi.org/10.4269/ajtmh.18-0622

APA

Ron-Roman, J., Ron-Garrido, L., Abatih, E., Celi-Erazo, M., Vizcaino-Ordonez, L., Calva-Pacheco, J., ... Saegerman, C. (2019). Bayesian evaluation of three serological tests for detecting antibodies against Brucella spp. among humans in the northwestern part of Ecuador. American Journal of Tropical Medicine and Hygiene, 100(6), 1312-1320. https://doi.org/10.4269/ajtmh.18-0622

Vancouver

Ron-Roman J, Ron-Garrido L, Abatih E, Celi-Erazo M, Vizcaino-Ordonez L, Calva-Pacheco J et al. Bayesian evaluation of three serological tests for detecting antibodies against Brucella spp. among humans in the northwestern part of Ecuador. American Journal of Tropical Medicine and Hygiene. 2019;100(6):1312-1320. https://doi.org/10.4269/ajtmh.18-0622

Author

Ron-Roman, Jorge ; Ron-Garrido, Lenin ; Abatih, Emmanuel ; Celi-Erazo, Maritza ; Vizcaino-Ordonez, Laura ; Calva-Pacheco, Jaime ; Gonzalez-Andrade, Pablo ; Berkvens, Dirk ; Benitez-Ortiz, Washington ; Brandt, Jef ; Fretin, David ; Saegerman, Claude. / Bayesian evaluation of three serological tests for detecting antibodies against Brucella spp. among humans in the northwestern part of Ecuador. In: American Journal of Tropical Medicine and Hygiene. 2019 ; Vol. 100, Nr. 6. blz. 1312-1320.

BibTeX

@article{6a14782072ab4fd4bf610ba32d832d8c,
title = "Bayesian evaluation of three serological tests for detecting antibodies against Brucella spp. among humans in the northwestern part of Ecuador",
abstract = "Brucellosis is an important but neglected zoonosis that causes serious economic losses both in livestock and human populations. The aim of the present study was to estimate the true prevalence of brucellosis together with diagnostic sensitivity and specificity of three serological tests in humans of the northwestern part of Ecuador using a Bayesian approach adjusted for the dependencies among the multiple tests to avoid any misinterpretation. In addition, the causal agent responsible for human brucellosiswas also identified. Using a total of 3,733 samples collected from humans in this area between 2006 and 2008, the prevalence of human brucellosis and the diagnostic test characteristics of the Rose Bengal fast agglutination test (RBT), Wright's slow agglutination test with ethylenediaminetetraacetic acid disodium salt dehydrate (EDTA) (SAT-EDTA), and indirect ELISA (iELISA) were estimated using a Bayesian approach. The estimated true prevalence of human brucellosis was 1{\%}(credibility interval: 0.4-1.6). The sensitivities of iELISA and RBT were higher than and similar (95.1{\%} and 95.0{\%}, respectively) to those of SAT-EDTA (60.8{\%}). Even though all tests indicated a high specificity (>99.0{\%}), the specificity of SAT-EDTAwas highest (99.9{\%}). The circulating strain in this study area was identified to be Brucella abortus biotype 4 based on culture and microbiological characterization. The RBT and the iELISA are recommended for estimating the true prevalence of human brucellosis and/or for surveillance programs following their high sensitivities and specificities. The proposed strategy supports evidence-based medicine for clinicians and policy-makers to ensure appropriate preventive and control program of brucellosis worldwide.",
keywords = "CONDITIONAL DEPENDENCE, DISEASE PREVALENCE, AGGLUTINATION-TEST, DIAGNOSIS, SPECIFICITY, SENSITIVITY, ELISA, SEROPREVALENCE, MELITENSIS, ABORTUS",
author = "Jorge Ron-Roman and Lenin Ron-Garrido and Emmanuel Abatih and Maritza Celi-Erazo and Laura Vizcaino-Ordonez and Jaime Calva-Pacheco and Pablo Gonzalez-Andrade and Dirk Berkvens and Washington Benitez-Ortiz and Jef Brandt and David Fretin and Claude Saegerman",
note = "CPDF; CINTEXT",
year = "2019",
doi = "10.4269/ajtmh.18-0622",
language = "English",
volume = "100",
pages = "1312--1320",
journal = "American Journal of Tropical Medicine and Hygiene",
issn = "0002-9637",
publisher = "AMER SOC TROP MED & HYGIENE",
number = "6",

}

RIS

TY - JOUR

T1 - Bayesian evaluation of three serological tests for detecting antibodies against Brucella spp. among humans in the northwestern part of Ecuador

AU - Ron-Roman, Jorge

AU - Ron-Garrido, Lenin

AU - Abatih, Emmanuel

AU - Celi-Erazo, Maritza

AU - Vizcaino-Ordonez, Laura

AU - Calva-Pacheco, Jaime

AU - Gonzalez-Andrade, Pablo

AU - Berkvens, Dirk

AU - Benitez-Ortiz, Washington

AU - Brandt, Jef

AU - Fretin, David

AU - Saegerman, Claude

N1 - CPDF; CINTEXT

PY - 2019

Y1 - 2019

N2 - Brucellosis is an important but neglected zoonosis that causes serious economic losses both in livestock and human populations. The aim of the present study was to estimate the true prevalence of brucellosis together with diagnostic sensitivity and specificity of three serological tests in humans of the northwestern part of Ecuador using a Bayesian approach adjusted for the dependencies among the multiple tests to avoid any misinterpretation. In addition, the causal agent responsible for human brucellosiswas also identified. Using a total of 3,733 samples collected from humans in this area between 2006 and 2008, the prevalence of human brucellosis and the diagnostic test characteristics of the Rose Bengal fast agglutination test (RBT), Wright's slow agglutination test with ethylenediaminetetraacetic acid disodium salt dehydrate (EDTA) (SAT-EDTA), and indirect ELISA (iELISA) were estimated using a Bayesian approach. The estimated true prevalence of human brucellosis was 1%(credibility interval: 0.4-1.6). The sensitivities of iELISA and RBT were higher than and similar (95.1% and 95.0%, respectively) to those of SAT-EDTA (60.8%). Even though all tests indicated a high specificity (>99.0%), the specificity of SAT-EDTAwas highest (99.9%). The circulating strain in this study area was identified to be Brucella abortus biotype 4 based on culture and microbiological characterization. The RBT and the iELISA are recommended for estimating the true prevalence of human brucellosis and/or for surveillance programs following their high sensitivities and specificities. The proposed strategy supports evidence-based medicine for clinicians and policy-makers to ensure appropriate preventive and control program of brucellosis worldwide.

AB - Brucellosis is an important but neglected zoonosis that causes serious economic losses both in livestock and human populations. The aim of the present study was to estimate the true prevalence of brucellosis together with diagnostic sensitivity and specificity of three serological tests in humans of the northwestern part of Ecuador using a Bayesian approach adjusted for the dependencies among the multiple tests to avoid any misinterpretation. In addition, the causal agent responsible for human brucellosiswas also identified. Using a total of 3,733 samples collected from humans in this area between 2006 and 2008, the prevalence of human brucellosis and the diagnostic test characteristics of the Rose Bengal fast agglutination test (RBT), Wright's slow agglutination test with ethylenediaminetetraacetic acid disodium salt dehydrate (EDTA) (SAT-EDTA), and indirect ELISA (iELISA) were estimated using a Bayesian approach. The estimated true prevalence of human brucellosis was 1%(credibility interval: 0.4-1.6). The sensitivities of iELISA and RBT were higher than and similar (95.1% and 95.0%, respectively) to those of SAT-EDTA (60.8%). Even though all tests indicated a high specificity (>99.0%), the specificity of SAT-EDTAwas highest (99.9%). The circulating strain in this study area was identified to be Brucella abortus biotype 4 based on culture and microbiological characterization. The RBT and the iELISA are recommended for estimating the true prevalence of human brucellosis and/or for surveillance programs following their high sensitivities and specificities. The proposed strategy supports evidence-based medicine for clinicians and policy-makers to ensure appropriate preventive and control program of brucellosis worldwide.

KW - CONDITIONAL DEPENDENCE

KW - DISEASE PREVALENCE

KW - AGGLUTINATION-TEST

KW - DIAGNOSIS

KW - SPECIFICITY

KW - SENSITIVITY

KW - ELISA

KW - SEROPREVALENCE

KW - MELITENSIS

KW - ABORTUS

U2 - 10.4269/ajtmh.18-0622

DO - 10.4269/ajtmh.18-0622

M3 - A1: Web of Science-article

VL - 100

SP - 1312

EP - 1320

JO - American Journal of Tropical Medicine and Hygiene

T2 - American Journal of Tropical Medicine and Hygiene

JF - American Journal of Tropical Medicine and Hygiene

SN - 0002-9637

IS - 6

ER -

ID: 3015009