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Reporter lines based on the gexp02 promoter enable early quantification of sexual conversion rates in the malaria parasite Plasmodium falciparum. / Portugaliza, Harvie P.; Llora-Batlle, Oriol; Rosanas-Urgell, Anna; Cortes, Alfred.

In: Scientific Reports, Vol. 9, No. 1, 14595, 2019, p. 14595.

Research output: Contribution to journalA1: Web of Science-article

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@article{6011cfb4270e432784c0969607b0da9f,
title = "Reporter lines based on the gexp02 promoter enable early quantification of sexual conversion rates in the malaria parasite Plasmodium falciparum",
abstract = "Transmission of malaria parasites from humans to mosquito vectors requires that some asexual parasites differentiate into sexual forms termed gametocytes. The balance between proliferation in the same host and conversion into transmission forms can be altered by the conditions of the environment. The ability to accurately measure the rate of sexual conversion under different conditions is essential for research addressing the mechanisms underlying sexual conversion, and to assess the impact of environmental factors. Here we describe new Plasmodium falciparum transgenic lines with genomeinte-grated constructs in which a fluorescent reporter is expressed under the control of the promoter of the gexp02 gene. Using these parasite lines, we developed a sexual conversion assay that shortens considerably the time needed for an accurate determination of sexual conversion rates, and dispenses the need to add chemicals to inhibit parasite replication. Furthermore, we demonstrate that gexp02 is expressed specifically in sexual parasites, with expression starting as early as the sexual ring stage, which makes it a candidate marker for circulating sexual rings in epidemiological studies.",
keywords = "TRANSMISSION, COMMITMENT, GAMETOCYTOGENESIS, GAMETOCYTES, EXPRESSION, DIFFERENTIATION, IDENTIFICATION, CULTURE, GENES, ASSAY",
author = "Portugaliza, {Harvie P.} and Oriol Llora-Batlle and Anna Rosanas-Urgell and Alfred Cortes",
note = "CPDF",
year = "2019",
doi = "10.1038/s41598-019-50768-y",
language = "English",
volume = "9",
pages = "14595",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

RIS

TY - JOUR

T1 - Reporter lines based on the gexp02 promoter enable early quantification of sexual conversion rates in the malaria parasite Plasmodium falciparum

AU - Portugaliza, Harvie P.

AU - Llora-Batlle, Oriol

AU - Rosanas-Urgell, Anna

AU - Cortes, Alfred

N1 - CPDF

PY - 2019

Y1 - 2019

N2 - Transmission of malaria parasites from humans to mosquito vectors requires that some asexual parasites differentiate into sexual forms termed gametocytes. The balance between proliferation in the same host and conversion into transmission forms can be altered by the conditions of the environment. The ability to accurately measure the rate of sexual conversion under different conditions is essential for research addressing the mechanisms underlying sexual conversion, and to assess the impact of environmental factors. Here we describe new Plasmodium falciparum transgenic lines with genomeinte-grated constructs in which a fluorescent reporter is expressed under the control of the promoter of the gexp02 gene. Using these parasite lines, we developed a sexual conversion assay that shortens considerably the time needed for an accurate determination of sexual conversion rates, and dispenses the need to add chemicals to inhibit parasite replication. Furthermore, we demonstrate that gexp02 is expressed specifically in sexual parasites, with expression starting as early as the sexual ring stage, which makes it a candidate marker for circulating sexual rings in epidemiological studies.

AB - Transmission of malaria parasites from humans to mosquito vectors requires that some asexual parasites differentiate into sexual forms termed gametocytes. The balance between proliferation in the same host and conversion into transmission forms can be altered by the conditions of the environment. The ability to accurately measure the rate of sexual conversion under different conditions is essential for research addressing the mechanisms underlying sexual conversion, and to assess the impact of environmental factors. Here we describe new Plasmodium falciparum transgenic lines with genomeinte-grated constructs in which a fluorescent reporter is expressed under the control of the promoter of the gexp02 gene. Using these parasite lines, we developed a sexual conversion assay that shortens considerably the time needed for an accurate determination of sexual conversion rates, and dispenses the need to add chemicals to inhibit parasite replication. Furthermore, we demonstrate that gexp02 is expressed specifically in sexual parasites, with expression starting as early as the sexual ring stage, which makes it a candidate marker for circulating sexual rings in epidemiological studies.

KW - TRANSMISSION

KW - COMMITMENT

KW - GAMETOCYTOGENESIS

KW - GAMETOCYTES

KW - EXPRESSION

KW - DIFFERENTIATION

KW - IDENTIFICATION

KW - CULTURE

KW - GENES

KW - ASSAY

U2 - 10.1038/s41598-019-50768-y

DO - 10.1038/s41598-019-50768-y

M3 - A1: Web of Science-article

C2 - 31601834

VL - 9

SP - 14595

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 14595

ER -

ID: 3080956