• Djamila Boushaki
  • Amel Adel
  • Mamadou Lamine Dia
  • Philippe Büscher
  • Hafsa Madani
  • Brahim Aymard Brihoum
  • Hassiba Sadaoui
  • Nadera Bouayed
  • Nadia Kechemir Issad

An epidemiological study of Trypanosoma evansi (T. evansi) infection in dromedaries was conducted in four wilayate (localities) of Southern Algeria: Bechar, El Bayadh, Ouargla, Tamanrasset. Between February 2014 and April 2016, 1056 camels of different ages and both sexes from 84 herds were sampled. The prevalence was determined through parasitological examination (Giemsa stained thin smear, GST), serological tests (CATT/T. evansi, ELISA/VSG RoTat 1.2, immune trypanolysis), and molecular tests (T. evansi type A specific RoTat 1.2 PCR and T. evansi type B specific EVAB PCR).

The overall prevalence was 2.4 % with GST, 32.4% with CATT/T. evansi, 23.1% with ELISA/VSG RoTat 1.2, 21.0% with immune trypanolysis (TL), 11.2 % with RoTat 1.2 PCR and 0% with EVAB PCR.

El Bayadh was the most affected wilaya with 11.8% positives in GST, 74.9% in CATT/T. evansi, 70.1% in ELISA/VSG RoTat 1.2 and 62.2% in immune trypanolysis. Only in Bechar, a non-significantly higher prevalence (13.6%) was observed with RoTat1.2 PCR than in El Bayadh (13.0%). We didn't find any evidence of the presence of T. evansi type B in the study area.

Original languageEnglish
Article number02086
JournalHeliyon
Volume5
Issue number7
Number of pages8
ISSN2405-8440
DOIs
Publication statusPublished - 2019

Bibliographical note

CPDF

    Research areas

  • Zoology, Algeria, Trypanosoma evansi, Surra, Dromedary camel, GST, RoTat 1.2 PCR, CATT/T. evansi, ELISA/VSG RoTat 1.2, Immune trypanolysis, SEROLOGICAL TESTS, HIGH AGREEMENT, LOW KAPPA, DIAGNOSIS, EXPRESSION, PREVALENCE, RESISTANCE, MOROCCO, ANTIGEN, DRUG

ID: 3026806