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Electrochemical detection of Toxocara canis excretory-secretory antigens in children from rural communities in Esmeraldas Province, Ecuador: association between active infection and high eosinophilia. / Morales-Yanez, Francisco; Trashin, Stanislav; Sariego, Idalia; Roucher, Clementine; Paredis, Linda; Chico, Martha; De Wael, Karolien; Muyldermans, Serge; Cooper, Philip; Polman, Katja.

In: Parasites and Vectors, Vol. 13, No. 1, 245, 2020, p. 245.

Research output: Contribution to journalA1: Web of Science-article

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@article{a82f669ddb8b42d199d57a81e3086c6e,
title = "Electrochemical detection of Toxocara canis excretory-secretory antigens in children from rural communities in Esmeraldas Province, Ecuador: association between active infection and high eosinophilia",
abstract = "Background The diagnosis of active Toxocara canis infections in humans is challenging. Larval stages of T. canis do not replicate in human tissues and disease may result from infection with a single T. canis larva. Recently, we developed a nanobody-based electrochemical magnetosensor assay with superior sensitivity to detect T. canis excretory-secretory (TES) antigens. Here, we evaluate the performance of the assay in children from an Ecuadorian birth cohort that followed children to five years of age. Methods Samples were selected based on the presence of peripheral blood eosinophilia and relative eosinophil counts. The samples were analyzed by the nanobody-based electrochemical magnetosensor assay, which utilizes a bivalent biotinylated nanobody as capturing agent on the surface of streptavidin pre-coated paramagnetic beads. Detection was performed by a different nanobody chemically labelled with horseradish peroxidase. Results Of 87 samples tested, 33 (38{\%}) scored positive for TES antigen recognition by the electrochemical magnetosensor assay. The average concentration of TES antigen in serum was 2.1 ng/ml (SD = 1.1). The positive result in the electrochemical assay was associated with eosinophilia > 19{\%} (P = 0.001). Parasitological data were available for 57 samples. There was no significant association between positivity by the electrochemical assay and the presence of other soil-transmitted helminth infections. Conclusions Our nanobody-based electrochemical assay provides highly sensitive quantification of TES antigens in serum and has potential as a valuable tool for the diagnosis of active human toxocariasis.",
keywords = "Toxocara, Eosinophilia, Nanobodies, Electrochemical assay, DIAGNOSIS, ANTIBODY, PREVALENCE",
author = "Francisco Morales-Yanez and Stanislav Trashin and Idalia Sariego and Clementine Roucher and Linda Paredis and Martha Chico and {De Wael}, Karolien and Serge Muyldermans and Philip Cooper and Katja Polman",
note = "CPDF",
year = "2020",
doi = "10.1186/s13071-020-04113-2",
language = "English",
volume = "13",
pages = "245",
journal = "Parasites and Vectors",
issn = "1756-3305",
publisher = "BMC",
number = "1",

}

RIS

TY - JOUR

T1 - Electrochemical detection of Toxocara canis excretory-secretory antigens in children from rural communities in Esmeraldas Province, Ecuador: association between active infection and high eosinophilia

AU - Morales-Yanez, Francisco

AU - Trashin, Stanislav

AU - Sariego, Idalia

AU - Roucher, Clementine

AU - Paredis, Linda

AU - Chico, Martha

AU - De Wael, Karolien

AU - Muyldermans, Serge

AU - Cooper, Philip

AU - Polman, Katja

N1 - CPDF

PY - 2020

Y1 - 2020

N2 - Background The diagnosis of active Toxocara canis infections in humans is challenging. Larval stages of T. canis do not replicate in human tissues and disease may result from infection with a single T. canis larva. Recently, we developed a nanobody-based electrochemical magnetosensor assay with superior sensitivity to detect T. canis excretory-secretory (TES) antigens. Here, we evaluate the performance of the assay in children from an Ecuadorian birth cohort that followed children to five years of age. Methods Samples were selected based on the presence of peripheral blood eosinophilia and relative eosinophil counts. The samples were analyzed by the nanobody-based electrochemical magnetosensor assay, which utilizes a bivalent biotinylated nanobody as capturing agent on the surface of streptavidin pre-coated paramagnetic beads. Detection was performed by a different nanobody chemically labelled with horseradish peroxidase. Results Of 87 samples tested, 33 (38%) scored positive for TES antigen recognition by the electrochemical magnetosensor assay. The average concentration of TES antigen in serum was 2.1 ng/ml (SD = 1.1). The positive result in the electrochemical assay was associated with eosinophilia > 19% (P = 0.001). Parasitological data were available for 57 samples. There was no significant association between positivity by the electrochemical assay and the presence of other soil-transmitted helminth infections. Conclusions Our nanobody-based electrochemical assay provides highly sensitive quantification of TES antigens in serum and has potential as a valuable tool for the diagnosis of active human toxocariasis.

AB - Background The diagnosis of active Toxocara canis infections in humans is challenging. Larval stages of T. canis do not replicate in human tissues and disease may result from infection with a single T. canis larva. Recently, we developed a nanobody-based electrochemical magnetosensor assay with superior sensitivity to detect T. canis excretory-secretory (TES) antigens. Here, we evaluate the performance of the assay in children from an Ecuadorian birth cohort that followed children to five years of age. Methods Samples were selected based on the presence of peripheral blood eosinophilia and relative eosinophil counts. The samples were analyzed by the nanobody-based electrochemical magnetosensor assay, which utilizes a bivalent biotinylated nanobody as capturing agent on the surface of streptavidin pre-coated paramagnetic beads. Detection was performed by a different nanobody chemically labelled with horseradish peroxidase. Results Of 87 samples tested, 33 (38%) scored positive for TES antigen recognition by the electrochemical magnetosensor assay. The average concentration of TES antigen in serum was 2.1 ng/ml (SD = 1.1). The positive result in the electrochemical assay was associated with eosinophilia > 19% (P = 0.001). Parasitological data were available for 57 samples. There was no significant association between positivity by the electrochemical assay and the presence of other soil-transmitted helminth infections. Conclusions Our nanobody-based electrochemical assay provides highly sensitive quantification of TES antigens in serum and has potential as a valuable tool for the diagnosis of active human toxocariasis.

KW - Toxocara

KW - Eosinophilia

KW - Nanobodies

KW - Electrochemical assay

KW - DIAGNOSIS

KW - ANTIBODY

KW - PREVALENCE

U2 - 10.1186/s13071-020-04113-2

DO - 10.1186/s13071-020-04113-2

M3 - A1: Web of Science-article

C2 - 32398157

VL - 13

SP - 245

JO - Parasites and Vectors

JF - Parasites and Vectors

SN - 1756-3305

IS - 1

M1 - 245

ER -

ID: 3397230