• Jaya Chakravarty
  • Sangeeta Kansal
  • Om Prakash Singh
  • Abhishek Kumar Singh
  • Ankita Chourasia
  • Toolika Singh
  • Medhavi Sudarshan
  • Akhil Pratap Singh
  • Bhawana Singh
  • Rudra Pratap Singh
  • Michaela Fakiola
  • Albert Picado
  • Jenefer M Blackwell
  • Mary E Wilson
  • David Sacks
  • Shyam Sundar

BACKGROUND: Asymptomatic Leishmania donovani infections outnumber clinical presentations, however the predictors for development of active disease are not well known. We aimed to identify serological, immunological and genetic markers for progression from L. donovani infection to clinical Visceral Leishmaniasis (VL).

METHODS: We enrolled all residents >2 years of age in 27 VL endemic villages in Bihar (India). Blood samples collected on filter paper on two occasions 6-12 months apart, were tested for antibodies against L. donovani with rK39-ELISA and DAT. Sero converters, (negative for both tests in the first round but positive on either of the two during the second round) and controls (negative on both tests on both occasions) were followed for three years. At the start of follow-up venous blood was collected for the following tests: DAT, rK39- ELISA, Quantiferon assay, SNP/HLA genotyping and L.donovani specific quantitative PCR.

RESULTS: Among 1,606 subjects enrolled,17 (8/476 seroconverters and 9/1,130 controls) developed VL (OR 3.1; 95% CI 1.1-8.3). High DAT and rK39 ELISA antibody titers as well as positive qPCR were strongly and significantly associated with progression from seroconversion to VL with odds ratios of 19.1, 30.3 and 20.9 respectively. Most VL cases arose early (median 5 months) during follow-up.

CONCLUSION: We confirmed the strong association between high DAT and/or rK39 titers and progression to disease among asymptomatic subjects and identified qPCR as an additional predictor. Low predictive values do not warrant prophylactic treatment but as most progressed to VL early during follow-up, careful oberservation of these subjects for at least 6 months is indicated.

Original languageEnglish
JournalPLoS Neglected Tropical Diseases
Volume13
Issue number3
Pages (from-to)e0007216
ISSN1935-2727
DOIs
Publication statusPublished - 2019

Bibliographical note

CPDF

    Research areas

  • Antibodies, Protozoan/blood, Asymptomatic Infections/epidemiology, Cohort Studies, Disease Progression, Endemic Diseases, Enzyme-Linked Immunosorbent Assay, Female, Humans, India/epidemiology, Infant, Leishmania donovani/genetics, Leishmaniasis, Visceral/epidemiology, Male, Seroconversion

ID: 3234499