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Comparative genomics shows differences in the electron transport and carbon metabolic pathways of Mycobacterium africanum relative to Mycobacterium tuberculosis and suggests an adaptation to low oxygen tension. / Ofori-Anyinam, Boatema; Riley, Abi Janet; Jobarteh, Tijan; Gitteh, Ensa; Sarr, Binta; Faal-Jawara, Tutty Isatou; Rigouts, Leen; Senghore, Madikay; Kehinde, Aderemi; Onyejepu, Nneka; Antonio, Martin; de Jong, Bouke C; Gehre, Florian; Meehan, Conor J.

In: Tuberculosis, Vol. 120, 101899, 2020.

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Ofori-Anyinam, Boatema ; Riley, Abi Janet ; Jobarteh, Tijan ; Gitteh, Ensa ; Sarr, Binta ; Faal-Jawara, Tutty Isatou ; Rigouts, Leen ; Senghore, Madikay ; Kehinde, Aderemi ; Onyejepu, Nneka ; Antonio, Martin ; de Jong, Bouke C ; Gehre, Florian ; Meehan, Conor J. / Comparative genomics shows differences in the electron transport and carbon metabolic pathways of Mycobacterium africanum relative to Mycobacterium tuberculosis and suggests an adaptation to low oxygen tension. In: Tuberculosis. 2020 ; Vol. 120.

BibTeX

@article{c88ae74ce3a74c2cabbb483ebbd275f5,
title = "Comparative genomics shows differences in the electron transport and carbon metabolic pathways of Mycobacterium africanum relative to Mycobacterium tuberculosis and suggests an adaptation to low oxygen tension",
abstract = "The geographically restricted Mycobacterium africanum lineages (MAF) are primarily found in West Africa, where they account for a significant proportion of tuberculosis. Despite this phenomenon, little is known about the co-evolution of these ancient lineages with West Africans. MAF and M. tuberculosis sensu stricto lineages (MTB) differ in their clinical, in vitro and in vivo characteristics for reasons not fully understood. Therefore, we compared genomes of 289 MAF and 205 MTB clinical isolates from the 6 main human-adapted M. tuberculosis complex lineages, for mutations in their Electron Transport Chain and Central Carbon Metabolic pathway in order to explain these metabolic differences. Furthermore, we determined, in silico, whether each mutation could affect the function of genes encoding enzymes in these pathways. We found more mutations with the potential to affect enzymes in these pathways in MAF lineages compared to MTB lineages. We also found that similar mutations occurred in these pathways between MAF and some MTB lineages. Generally, our findings show further differences between MAF and MTB lineages that may have contributed to the MAF clinical and growth phenotype and indicate potential adaptation of MAF lineages to a distinct ecological niche, which we suggest includes areas characterized by low oxygen tension.",
author = "Boatema Ofori-Anyinam and Riley, {Abi Janet} and Tijan Jobarteh and Ensa Gitteh and Binta Sarr and Faal-Jawara, {Tutty Isatou} and Leen Rigouts and Madikay Senghore and Aderemi Kehinde and Nneka Onyejepu and Martin Antonio and {de Jong}, {Bouke C} and Florian Gehre and Meehan, {Conor J}",
note = "CPDF; Copyright {\circledC} 2020 The Authors. Published by Elsevier Ltd.. All rights reserved.",
year = "2020",
doi = "10.1016/j.tube.2020.101899",
language = "English",
volume = "120",
journal = "Tuberculosis",
issn = "1472-9792",
publisher = "Churchill Livingstone",

}

RIS

TY - JOUR

T1 - Comparative genomics shows differences in the electron transport and carbon metabolic pathways of Mycobacterium africanum relative to Mycobacterium tuberculosis and suggests an adaptation to low oxygen tension

AU - Ofori-Anyinam, Boatema

AU - Riley, Abi Janet

AU - Jobarteh, Tijan

AU - Gitteh, Ensa

AU - Sarr, Binta

AU - Faal-Jawara, Tutty Isatou

AU - Rigouts, Leen

AU - Senghore, Madikay

AU - Kehinde, Aderemi

AU - Onyejepu, Nneka

AU - Antonio, Martin

AU - de Jong, Bouke C

AU - Gehre, Florian

AU - Meehan, Conor J

N1 - CPDF; Copyright © 2020 The Authors. Published by Elsevier Ltd.. All rights reserved.

PY - 2020

Y1 - 2020

N2 - The geographically restricted Mycobacterium africanum lineages (MAF) are primarily found in West Africa, where they account for a significant proportion of tuberculosis. Despite this phenomenon, little is known about the co-evolution of these ancient lineages with West Africans. MAF and M. tuberculosis sensu stricto lineages (MTB) differ in their clinical, in vitro and in vivo characteristics for reasons not fully understood. Therefore, we compared genomes of 289 MAF and 205 MTB clinical isolates from the 6 main human-adapted M. tuberculosis complex lineages, for mutations in their Electron Transport Chain and Central Carbon Metabolic pathway in order to explain these metabolic differences. Furthermore, we determined, in silico, whether each mutation could affect the function of genes encoding enzymes in these pathways. We found more mutations with the potential to affect enzymes in these pathways in MAF lineages compared to MTB lineages. We also found that similar mutations occurred in these pathways between MAF and some MTB lineages. Generally, our findings show further differences between MAF and MTB lineages that may have contributed to the MAF clinical and growth phenotype and indicate potential adaptation of MAF lineages to a distinct ecological niche, which we suggest includes areas characterized by low oxygen tension.

AB - The geographically restricted Mycobacterium africanum lineages (MAF) are primarily found in West Africa, where they account for a significant proportion of tuberculosis. Despite this phenomenon, little is known about the co-evolution of these ancient lineages with West Africans. MAF and M. tuberculosis sensu stricto lineages (MTB) differ in their clinical, in vitro and in vivo characteristics for reasons not fully understood. Therefore, we compared genomes of 289 MAF and 205 MTB clinical isolates from the 6 main human-adapted M. tuberculosis complex lineages, for mutations in their Electron Transport Chain and Central Carbon Metabolic pathway in order to explain these metabolic differences. Furthermore, we determined, in silico, whether each mutation could affect the function of genes encoding enzymes in these pathways. We found more mutations with the potential to affect enzymes in these pathways in MAF lineages compared to MTB lineages. We also found that similar mutations occurred in these pathways between MAF and some MTB lineages. Generally, our findings show further differences between MAF and MTB lineages that may have contributed to the MAF clinical and growth phenotype and indicate potential adaptation of MAF lineages to a distinct ecological niche, which we suggest includes areas characterized by low oxygen tension.

U2 - 10.1016/j.tube.2020.101899

DO - 10.1016/j.tube.2020.101899

M3 - A1: Web of Science-article

C2 - 32090860

VL - 120

JO - Tuberculosis

JF - Tuberculosis

SN - 1472-9792

M1 - 101899

ER -

ID: 3307732