Serology is the most convenient method for detecting brucellosis but the efficient use of such tests in disease control requires evaluation of diagnostic performance and discriminative ability. The objective of this study was to assess the performance of the Rose Bengal test (RBT) and an indirect ELISA (iELISA) in diagnosing brucellosis in 995 serum samples collected from cattle in the Ivory Coast between 2005 and 2009. A Bayesian approach was used to evaluate the two tests by estimating their sensitivities and specificities. The correlation-adjusted sensitivity of the iELISA was estimated to be 96.1% (credibility interval [CrI], 92.7-99.8), whereas that of the RBT was 54.9% (CrI, 23.5-95.1). High correlation-adjusted specificities were found for both tests (95.0%; [CrI, 91.1-99.6] for the iELISA and 97.7%; [CrI, 95.3-99.4] for the RBT, respectively). The true prevalence of brucellosis was estimated from the serum samples to be 4.6% (95%; [CrI, 0.6-9.5]). The level of agreement between the two tests was evaluated using indices of agreement (n=995). Good agreement was found for negative results (96.6%; confidence interval [CI], 95.7-97.4), a finding supported by an estimated significant correlation of 0.37 (95%; CI, 0.01-0.73) within the sera testing negative. Agreement was lower for sera testing positive (52.2% CI: 41.9-62.5). The findings highlight the importance of using these two tests in combination as part of any brucellosis control programme.
Original languageEnglish
JournalVeterinary Journal
Volume195
Issue number1
Pages (from-to)114-120
Number of pages7
ISSN1090-0233
DOIs
Publication statusPublished - 2013

Bibliographical note

ITG-B1B; ITG-B2B; ITG-B6B; ITG-B7A; DBM; U-VENTO; U-VEPID; JIF; DOI; PDF; Abstract; DSPACE

    Research areas

  • Animal diseases, Bovine tuberculosis, Brucella abortus, Prevalence, Detection, Estimation, Diagnostics, Laboratory diagnosis, Performance, Evaluation, Rose Bengal Test (RBT), Indirect hemagglutination test, ELISA, Sensitivity, Specificity, Identification, Strains, Côte d'Ivoire, Africa-West

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